Latest technologies from King's Business

Sound Emulation Technologies (Cvetkovic portfolio)

kingsbusiness-info@kcl.ac.uk — Tue, 05 Mar 2013 04:23:35 GMT

Perceptual surround sound recording and rendering Researchers from King's College London have invented a portfolio of emulation technologies to record and render perceptual surround sounds using computationally efficient algorithms. These techniques are amenable to a wide range of acoustic applications including mobile gaming, augmented reality, broadcasting audio, and surround sound television. Patents have been granted or filed for each design. The technologies are listed in this document (see below) but you can also find further information through this link including downloadable ...

Early detection of vascular calcification

kingsbusiness-info@kcl.ac.uk — Mon, 22 Oct 2012 03:04:09 GMT

Detection of early-stage vascular calcification King’s Ref: 501/0554 Technology King’s researchers have developed a non-invasive and inexpensive diagnostic assay for clinical assessment of vascular calcification (VC). Specific components of the patient’s blood are isolated and rapidly analyzed for a panel of novel molecular markers indicative of early-stage VC. A clinical calcification risk and progression score is then calculated, thus facilitating early detection and treatment. The Problem VC is an increasingly important clinical concern, particularly for s...

Cardioplegia

kingsbusiness-info@kcl.ac.uk — Mon, 22 Oct 2012 03:03:43 GMT

St Thomas’ Hospital Polarised Cardioplegic Solution King’s ref: 501/0458 Technology A non-depolarising cardioplegic solution developed by St Thomas’ Hospital and King’s College London for use in cardiac surgery with the advantageous properties of fast induction of cardiac arrest, slow onset of global ischemia, low systemic toxicity and rapid reversibility and clearance from the body. Background During cardiac surgery, the heart is subjected to an elective period of global ischemia to provide the surgeon with a blood-free operating field and a still, flaccid heart. ...

Diagnostic for Autism Spectrum Disorders

kingsbusiness-info@kcl.ac.uk — Fri, 17 Aug 2012 01:30:46 GMT

Technology King’s researchers have developed a highly accurate diagnostic for Autism Spectrum Disorders (ASD). The Problem Autism Spectrum Disorders (ASDs) are a spectrum of neurodevelopmental conditions characterized by impairments in social interaction and communication, and associated with repetitive, restricted patterns of interest or behaviour. ASD is an umbrella term used to describe a number of autism disorders such as classic autism, Asperger's Syndrome, atypical autism and pervasive developmental disorder not otherwise specified. It is well established that ASD a...

Modified Calcium Silicate-Containing Adhesive Systems

kingsbusiness-info@kcl.ac.uk — Mon, 14 May 2012 08:20:00 GMT

Technology King’s researchers in collaboration with the University of Bologna, Italy have developed innovative bioactive materials for direct resin-composite restorations, dentine hypersensitivity treatment, cavity liner for caries dentine. This material may also have a potential application in apical root, root canal and root perforation treatments. The Problem Simplified etch-and-rinse and/or self-etch adhesive systems currently used in conservative/restorative dentistry are affected by reduced longevity due to the degradation of the hybrid layer. The hybrid layer...

Anti-CSPG4 antibody for the treatment of melanoma

kingsbusiness-info@kcl.ac.uk — Wed, 09 May 2012 06:01:31 GMT

A chimeric IgE class anti-CSPG4 antibody has been developed which has demonstrated positive results in vitro and in vivo. The antibody is designed to target immune responses directly against melanoma tumour cells, circumventing the broad immune activatory properties of currently approved immunotherapies. Figure: CSPG4 IgE restricts growth of an A375 human melanoma xenograft grown subcutaneously compared to CSPG4 IgG1 (**p<0.01) and non-specific antibody isotype controls when administered intravenously in a humanised mouse model with splenic engraftment of human immu...

Flow Cytometry-based Non-Transferrin-Bound Iron Assay

kingsbusiness-info@kcl.ac.uk — Wed, 28 Sep 2011 03:54:59 GMT

Fe-Flobead: A Sensitive High Throughput Test For Iron Overload An iron-sensitive fluorescent probe attached to beads has been developed and incorporated into an assay for the sensitive and accurate measurement of non-transferrin-bound iron (NTBI). Measurement of NTBI should provide a valuable means of early detection and follow-up of ideseases associated with iron overload such as thalassaemia, sickle cell anaemia, myelodysplastic syndrome and haemochromatosis. Introduction NTBI refers to all forms of iron in the plasma that bind to ligands other than tran...

A method for reducing or eliminating non resonant background obtained during CARS spectroscopy

kingsbusiness-info@kcl.ac.uk — Wed, 23 Mar 2011 07:27:21 GMT

Coherent anti-Stokes Raman Scattering (CARS) spectroscopy can give chemically specific information on cell structures and processes. However non-resonant background signals (NRB) have limited the full potential of this technique in the study of biological systems. It is this problem of NRB in CARS spectroscopy that King’s researchers have addressed; using what is essentially an interferometric process to reduce the NRB signals. The intention was to develop a simpler apparatus for performing CARS, meaning that this novel approach to the removal of NRB is not relia...

Human Anti-GAD65 Monoclonal Antibodies - Clone b96.11

kingsbusiness-info@kcl.ac.uk — Fri, 21 May 2010 06:40:30 GMT

Reagent name Human monoclonal antibodies to GAD65 (clone b96.11) Immunogen No immunogen, as generated from a patient with polyendocrine autoimmune disease (APS2) Relevance Can be used as a positive control antibody in diagnostic kits to enumerate antibodies to GAD65 in type 1 diabetic patients. Autoantibodies to the 65-kDa isoform of glutamic acid decarboxylase (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome (SMS), and polyendocrine autoimmune disease. Autoantibodies to GAD65 are important mar...

Human Anti-GAD65 Monoclonal Antibodies - Clone b78

kingsbusiness-info@kcl.ac.uk — Fri, 21 May 2010 06:40:30 GMT

Reagent Name Human monoclonal antibodies to GAD65 (clone b78) Immunogen No immunogen, as generated from a patient with polyendocrine autoimmune disease (APS2) Relevance Can be used as a positive control antibody in diagnostic kits to enumerate antibodies to GAD65 in type 1 diabetic patients. Autoantibodies to the 65-kDa isoform of glutamic acid decarboxylase (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome (SMS), and polyendocrine autoimmune disease. Autoantibodies to GAD65 are important marker...

HIV-1 integrase (IN) monoclonal antibody - Clone IN-2

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:35 GMT

Reagent name HIV-1 integrase (IN) monoclonal antibody (#IN-2) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 integrase protein (clade B, HXB-3 isolate) Relevance HIV-1 expresses the integrase (IN) protein as part of the polymerase gene. It is the viral enzyme that catalyses the insertion of viral DNA into the chromosomal DNA of an infected cells. Integrase is essential for viral growth and is a therapeutic target. Cross-reactivity ...

Mouse anti-human actin antibody - Clone MM2/193

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:32 GMT

Reagent Name Mouse anti-human actin antibody Immunogen Human platelet membranes. Relevance Actin is a major protein found in most cells and is important in cell motility and contraction. It is a major component of the cytoskeleton. Cross reactivity Recognises human actin as a 42-47 kDa protein in platelet lysates. Specificity Epitope is not mapped. Tested Applications Western blotting. Raised in Mouse Clonality Monoclonal

Human-APOBEC3G Polyclonal Antibody

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:31 GMT

APOBEC3G is a human protein with natural antiviral activity, especially against HIV. As such, it is currently the subject of intense scientific investigation, and may lead to the development of new therapeutics useful for treating HIV/AIDS. Indirect Immunofluorescence HeLa cell monolayers were transfected with an APOBEC3G expression vector, fixed after 24 hr and hybridised with the anti-APOBEC3G serum (1:500 dilution). Bound antibody was detected using an Alexa Fluor 594-conjugated goat secondary antibody and imaged by confocal microscopy. APOBEC3G is seen (...

Mouse anti- human filamin antibody - Clone PM6/317

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:28 GMT

Reagent Name Mouse anti- human filamin antibody Immunogen Human platelet membranes Relevance Filamin (also called actin binding protein) is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site. It is present in smooth muscle, fibroblasts, platelets and lymphocytes. Cross reactivity Chicken, Guinea Pig , Human, Rat, Rabbit Specificity Recognizes full length Human filamin (actin-binding protein; 270-280 kDa) as well as the 190...

HIV-1 p24 Gag monoclonal antibody - Clone 24-4

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:27 GMT

Reagent Name HIV-1 p24 Gag monoclonal antibody (#24-4) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p24 Gag protein (clade B, HXB-3 isolate) Relevance p24 is encoded by the HIV gag gene and is an important structural component of the capsid core of the HIV particle Cross reactivity Cross-reactivity with a ~65 kDa species in western blot Specificity Epitope not yet mapped Tested applications Western blots for clade B isolat...

HIV-1 p24 Gag monoclonal antibody - Clone 24-3

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:26 GMT

Reagent Name HIV-1 p24 Gag monoclonal antibody (#24-3) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p24 Gag protein (clade B, HXB-3 isolate) Relevance p24 is encoded by the HIV gag gene and is an important structural component of the capsid core of the HIV particle Cross reactivity Not tested Specificity Epitope not mapped Tested applications Western blots for clade B isolates of HIV; immunofluorescence assays. (not useful for...

Mouse anti-human Integrin beta 3 (CD61) antibody - Clone PM6/13

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:25 GMT

Reagent Name Human Integrin beta 3 antibody Immunogen Human platelet membranes Relevance Integrin beta 3 is also known as glycoprotein IIIa and in a complex with the integrin alpha 2b subunit forms the receptor for fibrinogen, fibronectin and von Willebrand Factor on activated platelets. Integrin beta 3 can also complex with alpha V integrin. The antibody inhibits platelet aggregation, inhibits the binding of soluble fibrinogen to activated platelets and also inhibits adhesion of activated platelets to immobilised fibrinogen, fibronectin or vWF. Cross rea...

Mouse anti-human Vinculin antibody - Clone V284

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:19 GMT

Reagent Name Mouse anti-human Vinculin antibody Immunogen Purified human platelet vinculin Relevance Vinculin is a 117 kDa membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. Cross reactivity Human, rabbit, rat, chicken, mouse. Specificity Epitope not mapped. Recognises vinculin and a 130 kDa protein in platelets and other cells. Tested Applications Western blotting, immunoprecipitation, immunohistology Raised in Mouse Clonality Monoclonal &nbs...

Optical endoscope for vascular imaging and concurrent oximetry instrument for non surgical tissue vascular assessment.

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:15 GMT

Technology A clinic based optical instrument that allows a doctor to examine the blood supply to a particular tissue, without need for surgical biopsy. Based on multiple wavelength analysis of light reflected by sub-cutaneous fatty layers, this instrument will allow high-resolution video-rate mapping of capillary blood flow and oxygen carriage from the same tissue simultaneously. This is not clinically possible with any instrument at present. Market Vascular imaging has applications in surgical, medical and research disciplines, such as transplant surgery and asses...

Bimodal Nanoparticles as biological labels

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:14 GMT

Novel, cadmium-free, fluorescent and magnetic nanoparticles suitable for surface functionalisation. Photographs of four different bimodal magnetic conjugated polymer nanoparticles, demonstrating their magnetic and fluorescent functionalities Summary King’s Inventors have developed a robust method for the synthesis of water soluble, multicoloured fluorescent-magnetic nanoparticles which do not involve the use of quantum dots, with their associated potential toxicity issues. These lipid encapsulated nanoparticles are made from well characterised, widely available conjugate...

Mouse anti-human integrin alpha 2b (CD41) antibody - Clone PM6/248

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:11 GMT

Reagent Name Mouse anti-human integrin alpha 2b (CD41) antibody Immunogen Human platelet membranes Relevance Integrin alpha 2b is also known as glycoprotein IIb and in a complex with the integrin beta 3 subunit forms the receptor for fibrinogen, fibronectin and von Willebrand Factor on activated platelets. The antibody activates platelets under stirring conditions and inhibits at higher doses . The antibody inhibits binding of fibrinogen, fibronectin and vWF in static adhesion assays and inhibits binding of soluble fibrinogen and fibronectin to ADP activated platelet...

Human Thyrotrophin Receptor Monoclonal Antibody - Clone 2C11

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:10 GMT

The human thyrotrophin receptor (TSHR) is found primarily in epithelial cells of thyroid follicles where it induces (by cAMP) the phospholipase C and protein kinase A signal transduction pathways. TSHR is the major pathogenic autoantigen in human autoimmune thyroid Graves’ disease. Lanes 1. TSHR 2. Glycosylated TSHR 3. TSHR - GST 4. Residues 125-369 TSHR - GST Product Details Product Type Hybridoma for monoclonal antibody Immunogen Bacterially expressed extracellular domain (residues 1-415 of human TSHR fused to GST) Cross Reactivity Cro...

Human Visual Arrestin MAb - Clone BDS-2

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:04 GMT

Research tool for • Immunoblotting • Immunohistochemistry • ELISA • Affinity purification • Blocking interacting proteins Visual arrestin (also known as S-Antigen) is a 48kDa protein expressed exclusively in retinal photoreceptor cells where it regulates light-dependent signal transduction through G protein-coupled receptor (rhodopsin) activation. Visual arrestin is the major pathogenetic autoantigen in human autoimmune ...

HIV-1 p17 Gag monoclonal antibody - Clone 17-1

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:02 GMT

Reagent Name HIV-1 p17 Gag monoclonal antibody (#17-1) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1. Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p17 Gag protein (clade B, HXB-3 isolate) Relevance p17 is encoded by the HIV gag gene and is an important structural component of the HIV particle Cross reactivity Not tested Specificity Epitope not mapped Tested applications Western blots for clade B isolates of HIV; immunoprecipitation assays; immunofluorescence/FACS assay...

Mouse Anti-T Cell / Neutrophil Marker Antibody - Clone RPN3/57

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:01 GMT

Reagent Name Mouse Anti-T Cell / Neutrophil Marker Antibody Immunogen Rabbit peritoneal neutrophils Relevance Useful for the detection of rabbit neutrophils and T cells Cross reactivity Not tested Specificity Recognises an antigen expressed strongly on neutrophils, thymocytes, a subset of T cells and platelets. Epitope not identified. Tested Applications Flow cytometry and immunohistochemistry Raised in Mouse Clonality Monoclonal Isotype IgG1 References 1) Wilkinson JM et al. (1992) Immunol. ...

Mouse anti-human SERCA 3 antibody - Clone PL/IM430

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:46:00 GMT

Reagent Name Mouse anti-human SERCA 3 antibody Immunogen Human platelet intracellular membranes Relevance SERCA 3 is the sarco-endoplasmic reticulum Ca2+ ATPase that sequesters Ca2+ into intracellular stores of mammalian cells. This antibody inhibits Ca2+ sequestration and Ca2+ATPase activity mediated by human SERCA3. Cross reactivity Human Specificity Epitope has recently been described to be between amino acids 1-236 of human SERCA3. PL/IM430 recognises a 100kDa SERCA3 protein in platelet lysates. Tested Applications Western blotting, i...

HIV-1 Rev monoclonal antibody - Clone Rev-4

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:59 GMT

Reagent Name HIV-1 Rev monoclonal antibody (#Rev-4) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 Rev protein (clade B, HXB-3 isolate) Relevance HIV-1 expresses the trans-activator protein Rev, which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. Cross-reactivity No cross-reactivity with non-HIV-1 Rev pr...

Human Complement C1q Monoclonal Antibody - Clone IA4

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:54 GMT

Research tool for: • Immunohistochemistry • Isolation of C1q complexes C1q is a sub-component of the first complement component C1. C1q binds to IgG and IgM immune complexes, this activates C1r and C1s, which are also bound to C1q and so initiates the classical pathway of complement activation. C1q deficiencies are observed in agammaglobulinemia, acute glomerular nephritis, systemic lupus erythematosus, and chronic glomerular nephritis. Product Details Product Type &...

Human-APOBEC3F Polyclonal Antibody

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:51 GMT

APOBEC3F is a human protein with natural antiviral activity, especially against HIV. As such, it is currently the subject of intense scientific investigation, and may lead to the development of new therapeutics useful for treating HIV/AIDS. Expression and virion incorporation of APOBEC3F proteins Immunoblot analysis of APOBEC3F wild-type (WT) and mutant proteins was performed on both 293T cells transfected with APOBEC3F expression plasmids and concentrated virus produced from these cells. Equivalent amounts of protein were loaded, as judged by amounts of the cellular prote...

HIV-1 Vif Monoclonal Antibody - Clone 319

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:50 GMT

Reagent Name HIV-1 Vif monoclonal antibody (#319) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 Vif protein (clade B, HXB-3 isolate) Relevance HIV-1 expresses the viral infectivity factor (vif) which induces productive viral replication by inactivating the host cell anti-HIV factor APOBEC3G Cross-reactivity No cross-reactivity with non-HIV-1 Vif proteins has been observed Specificity Epitope not yet mapped Tested application...

Human Visual Arrestin MAb - Clone PDS-1

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:49 GMT

HIV-1 p24 Gag monoclonal antibody - Clone 24-2

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:48 GMT

Reagent Name HIV-1 p24 Gag monoclonal antibody (#24-2) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p24 Gag protein (clade B, HXB-3 isolate) Relevance p24 is encoded by the HIV gag gene and is an important structural component of the capsid core of the HIV particle Cross reactivity Not tested Specificity Epitope not mapped Tested applications Western blots for clade B isolates of HIV; immunofluorescence assays; immunoprecipita...

Human Thyrotrophin Receptor Monoclonal Antibody - Clone 4C1

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:47 GMT

Research tool for • Western blotting • Immunoprecipitation • Flow cytometry • Immunohistochemistry (frozen section) Inhibits binding of TSH to TSH receptor by 70-80%. The human thyrotrophin receptor (TSHR) is found primarily in epithelial cells of thyroid follicles where it induces (by cAMP) the phospholipase C and protein kinase A signal transduction pathways. TSHR is the major pathogenic autoantigen in human autoimmune thyroid Graves’ diseas...

HIV-1 integrase (IN) monoclonal antibody - Clone IN-3

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:43 GMT

Reagent name HIV-1 integrase (IN) monoclonal antibody (#IN-3) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 integrase protein (clade B, HXB-3 isolate) Relevance HIV-1 expresses the integrase (IN) protein as part of the polymerase gene. It is the viral enzyme that catalyses the insertion of viral DNA into the chromosomal DNA of an infected cells. Integrase is essential for viral growth and is a therapeutic target. Cross-reactivity ...

Genes Related To Foetal Haemoglobin Expression

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:41 GMT

King’s researchers have discovered a number of important polymorphic markers or single nucleotide polymorphisms (SNP’s) associated with increased production of fetal haemoglobin (HbF) in an individual. Increased levels of HbF have been shown to have a positive effect on the severity of sickle cell disease and beta- thalassaemia and is the focus of several sickle cell disease therapeutics. Thus, these genetic markers can be used in an ante- or postnatal diagnostic to predict disease severity and help guide genetic counselling and the level and type of treatment needed to maintain long te...

Human Thyrotrophin Receptor Monoclonal Antibody - Clone 3B12

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:40 GMT

Research tool for: • Immunoblotting • Immunoprecipitation • Flow cytometry • Immunohistochemistry (frozen section) Product details Product Type Hybridoma for monoclonal antibody Immunogen Bacterially expressed extracellular domain (residues 1-415) of human TSHR fused to GST Cross Reactivity Cross-reacts with human and theoretically with rat, pig, sheep, mouse, dog, ...

HIV-1 p17 Gag monoclonal antibody - Clone 17-3

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:40 GMT

Reagent Name HIV-1 p17 Gag monoclonal antibody (#17-3) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p17 Gag protein (clade B, HXB-3 isolate) Relevance p17 is encoded by the HIV gag gene and is an important structural component of the HIV particle Cross reactivity Not tested Specificity Epitope not mapped Tested applications Western blots for clade B isolates of HIV; immunoprecipitation assays; immunofluorescence/FACS assay...

WFDC1/ps20, a novel marker for HIV infection

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:39 GMT

King’s inventors have identified a novel HIV enhancing factor, WFDC1/ps20, implicated in HIV pathogenesis. The need The two widely used clinical markers of HIV disease progression are: a declining peripheral blood CD4 T cell count and the level of plasma virus load (VL). Currently, a flow-cytometry based assay is used to measure CD4 T cell count and a PCR-based assay to measure plasma virus load. There are two major limitations to these assays: • Do not capture breadth of disease: It is increasingly clear that these markers do not capture the ...

HIV-1 Rev monoclonal antibody - Clone Rev-6

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:38 GMT

Reagent Name HIV-1 Rev monoclonal antibody (#Rev-6) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 Rev protein (clade B, HXB-3 isolate) Relevance HIV-1 expresses the trans-activator protein Rev, which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. Cross-reactivity No cross-reactivity with non-HIV-1 Rev pr...

HIV-1 Vif Monoclonal Antibody - Clone 564

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:37 GMT

Reagent Name HIV-1 Vif monoclonal antibody (#564) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 Vif protein (clade B, HXB-3 isolate) Relevance HIV-1 expresses the viral infectivity factor (vif) which induces productive viral replication by inactivating the host cell anti-HIV factor APOBEC3G Cross-reactivity No cross-reactivity with non-HIV-1 Vif proteins has been observed Specificity Epitope not yet mapped Tested applications Immuno...

Saliva based test to identify at risk women who will deliver pre-term

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:36 GMT

King’s College London and University College London researchers have identified a diagnostic marker found in saliva samples which can be used to identify women likely to deliver preterm. The saliva test could be used to predict very premature birth in pregnant women. The researchers have undertaken a small study in women whom they knew to be at risk of early delivery from their clinical history. Samples were provided by pregnant women taking part in a prospective randomised controlled trial (the PREMET study). The data suggest that measurement of progesterone in a s...

Mouse anti-human Talin antibody - Clone TA205

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:33 GMT

Reagent Name Mouse anti-human Talin antibody Immunogen Human platelet talin. Relevance Talin is a cytoskeletal protein that is found concentrated in focal adhesions. It is capable of linking integrins to the actin cytoskeleton either directly or indirectly by interacting with vinculin and alpha-actinin Cross reactivity TA205 immunoblots a 225 kDa protein in human platelet lysates Specificity Epitope resides in region 139-433 in talin. Tested Applications Immunohistochemistry, Western blotting and immunoprecipitation. Raised in Mouse Cl...

Mouse anti-human Glycoprotein 1b alpha (CD42b) antibody - Clone PM6/40.

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:30 GMT

Reagent Name Mouse anti-human Glycoprotein 1b alpha antibody Immunogen Platelet membranes Relevance Glycoprotein 1b (GP1b) is heavily expressed in platelets and megakaryocytes. It serves as a receptor for von Willebrand Factor and thrombin. Cross reactivity Recognises human GP1b, other species GP1b to be tested. Specificity Epitope not mapped Tested Applications Flow cytometry, immunoflourescence, immunohistochemistry (frozen sections) Raised in Mouse Clonality Monoclonal Isotype IgG1 References Thorsen LI et al 1998 Br. J...

Human Thyroglobulin Monoclonal Antibody - Clone 1D4

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:29 GMT

Research tool for • ELISA • Radioimmunoassay • In vivo imaging Thyroglobulin is a large iodinated glycoprotein which is produced by the thyroid gland, functioning as a prohormone in the synthesis of thyroxine (T4) and triiodothyronine (T3). Levels of serum thyroglobulin are elevated in a variety of thyroid diseases, such as Grave’s disease (goiter), subacute thyroiditis and differentiated thyroid carcinoma. Properties Product Type Hybridoma for monoclonal antibody I...

SuperBUGS: Rabbit anti-MAP1B phospho (T1265)

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:23 GMT

Affinity purified anti-sera to the glycogen synthase kinase (GSK3b) mediated nonprimed phosphorylation site of Microtubule Associated Protein 1B (MAP1B). MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. King’s Developer Professor Phillip R. Gordon-Weeks Reagent name SuperBUGS: Rabbit anti-MAP1B phospho (T1265) Immunogen (peptide sequence + location; recombinant protein; native protein from which species) CSPIEKTPLGER, in which threonine was phosphorylated, were synthesi...

Huntington’s disease mouse model - the gold standard

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:22 GMT

The Huntington’s Disease (HD) R6/2 transgenic mouse model, the gold standard for preclinical Huntington’s research, was developed by King’s Professor Gillian Bates, a world-leader in HD research. The R6/2 transgenic mouse model • Displays the major symptoms of Huntington’s disease and remains the most used model when testing HD therapeutics; • Licensed to 13 companies (including global pharmaceutical companies & clinical research organisations) for internal R&D programs; • Has...

Mouse anti-human Glycoprotein 1b alpha (CD42b) antibody - Clone MM2/174.

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:21 GMT

Reagent Name Mouse anti-human Glycoprotein 1b alpha (GP1b alpha subunit) antibody Immunogen Platelet membranes Relevance Glycoprotein 1b is heavily expressed in platelets and megakaryocytes. It serves as a receptor for von Willebrandt Factor and thrombin. Cross reactivity Recognises human GP1b, other species to be tested. Specificity Epitope not mapped. Tested Applications Western blotting, flow cytometry immunohistochemistry, immunofluorescence. Raised in Mouse Clonality Monoclonal Isotype IgG1 References Thorsen LI et al ...

Human PMP22 Monoclonal Antibody

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:17 GMT

The First Human MAb developed specific for PMP22. Research tool for: • Western blotting • Immunohistochemistry – paraffin sections • ELISA Peripheral Myelin Protein 22 (PMP22) is a 160 amino acid glycoprotein which is a member of the claudin family and is thought to play an important role in the structure of peripheral nerve myelin. Irregularities in its gene results in hereditary demyelinating neuropathies. PMP22 protein is present in the compact myelin of adult h...

HIV-1 p17 Gag monoclonal antibody - Clone 17-2

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:15 GMT

Reagent Name HIV-1 p17 Gag monoclonal antibody (#17-2) Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 p17 Gag protein (clade B, HXB-3 isolate) Relevance p17 is encoded by the HIV gag gene and is an important structural component of the HIV particle Cross reactivity Not tested Specificity Epitope not mapped Tested applications Western blots for clade B isolates of HIV Raised in Mouse Clonality Monoclonal ...

HIV-1 monoclonal antibodies

kingsbusiness-info@kcl.ac.uk — Wed, 14 Apr 2010 08:45:13 GMT

Reagent Name HIV-1 monoclonal antibodies Clones available Clone 17-1 for HIV-1 p17 Gag MAb Clone 17-2 for HIV-1 p17 Gag MAb Clone 17-3 for HIV-1 p17 Gag MAb Clone 24-3 for HIV-1 p24 Gag MAb Clone 24-4 for HIV-1 p24 Gag MAb Clone IN-2 for HIV-1 integrase (IN) MAb Clone IN-3 for HIV-1 integrase (IN) MAb Clone 564 for HIV-1 Vif MAb Clone 319 for HIV-1 Vif MAb Clone Rev-6 for HIV-1 Rev MAb Clone Rev-4 for HIV-1 Rev MAb Description Hybridoma expressing antibodies to human immunodeficiency virus type 1 Immunogen Bacterially expressed, hexahistidine amino-...